General
Preferred name
PFI-1
Synonyms
PFI 1 ()
PF-6405761 ()
PFI1 ()
QCR-192 ()
PFI-1 (PF-6405761) ()
P&D ID
PD000013
CAS
1403764-72-6
Tags
available
probe
drug candidate
Drug indication
Discovery agent
Probe info
Probe type
calculated probe
experimental probe
P&D approved
Probe selectivity
protein-selective
Probe sources
Bromodomains chemical toolbox
Chemical Probes.org
High-quality chemical probes
SGC Probes
Tool Compound Set
Probe targets
[[ compound.targets[t].gene_name ]]
Probe control
Probe control not defined
Orthogonal probes
22
No orthogonal probes found
Similar probes
0
No structurally similar probes found
Structure
P&D probe-likeness score
Structure formats
[[ format ]]
[[ compound[format === 'MOL' ? 'molblock' : format.toLowerCase()] ]]
Description
(extracted from source data)
COMMENT
This probe meets SGC probe criteria. Jun 16 2016 - 5:18pm; This probe meets minimal criteria for use in both cellular and in vivo assays. Plasma protein binding information relevant to its use: PFI-1 displayed an IC50 of 1.89 uM (n=6) in LPS-stimulated PBMCs with an IL-6 readout, using RPMI media containing 5% FBS (see PMID: 23095041). Jun 20 2016 - 9:27pm
DESCRIPTION
This compound is an inhibitor of the bromodomain and extra-terminal (BET) proteins, BRD2-4 . This is a compound from the Structural Genomics Consortium's (SGC) Epigenetics Probes Collection.
(GtoPdb)
DESCRIPTION
PFI-1 is a selective BET (bromodomain-containing protein) inhibitor for BRD4 with IC50 of 0.22 ¦ÌM in a cell-free assay.
PRICE
53
DESCRIPTION
PFI-1 (PF-6405761), a specific BET (bromodomain-containing protein) inhibitor for BRD4, is with the IC50 of 0.22 ??M in a cell-free assay.
MOA
Antagonist
(Chemical Probes.org)
DESCRIPTION
Kruppel-like factor 5 (KLF5) inhibitor
(Tocris Bioactive Compound Library)
DESCRIPTION
BET bromodomain inhibitor
(Tocriscreen Plus)
DESCRIPTION
PFI-1 is a potent and highly selective protein interaction Inhibitor, which targets BET bromodomain. PFI-1 that efficiently blocks the interaction of BET BRDs with acetylated histone tails. Co-crystal structures showed that PFI-1 acts as an acetyl-lysine (Kac) mimetic inhibitor efficiently occupying the Kac binding site in BRD4 and BRD2. PFI-1 has antiproliferative effects on leukaemic cell lines and efficiently abrogates their clonogenic growth. Exposure of sensitive cell lines with PFI-1 results in G1 cell cycle arrest, down-regulation of MYC expression as well as induction of apoptosis and induces differentiation of primary leukaemic blasts. Intriguingly, cells exposed to PFI-1 showed significant down-regulation of Aurora B kinase, thus attenuating phosphorylation of the Aurora substrate H3S10 providing an alternative strategy for the specific inhibition of this well established oncology target.
(BOC Sciences Bioactive Compounds)
DESCRIPTION
PFI-1 is a selective bromodomain-containing protein inhibitor.
(Enamine Bioactive Compounds)
DESCRIPTION
PFI-1 (PF-6405761), a specific BET (bromodomain-containing protein) inhibitor for BRD4, is with the IC50 of 0.22 μM in a cell-free assay.
(TargetMol Bioactive Compound Library)
[[ p.pathway_name ]]
[[ compound.targets[tid].gene_name ]]
Cell lines
62
Organisms
0
Compound Sets
28
AdooQ Bioactive Compound Library
Axon Medchem Screening Library
BOC Sciences Bioactive Compounds
Bromodomains chemical toolbox
Cayman Chemical Bioactives
Chemical Probes.org
CZ-OPENSCREEN Bioactive Library
Drug Repurposing Hub
DrugMAP
Enamine Bioactive Compounds
Enamine BioReference Compounds
EU-OPENSCREEN Bioactive Compound Library
EUbOPEN Chemogenomics Library
Guide to Pharmacology
High-quality chemical probes
JUMP-MOA Compound Set
JUMP-Target 1 Compound Set
LINCS compound set
Mcule NIBR MoA Box Subset
MedChem Express Bioactive Compound Library
Novartis Chemogenetic Library (NIBR MoA Box)
Selleckchem Bioactive Compound Library
SGC Probes
TargetMol Bioactive Compound Library
Tocris Bioactive Compound Library
Tocriscreen Plus
Tool Compound Set
Welcome Trust Cancer Drugs
[[ a.name ]]
[[ ligand_id ]]
free of charge
External IDs
20
Molecular Weight
347.09
Hydrogen Bond Acceptors
4
Hydrogen Bond Donors
2
Rotatable Bonds
4
Ring Count
3
Aromatic Ring Count
2
cLogP
2.47
TPSA
87.74
Fraction CSP3
0.19
Chiral centers
0.0
Largest ring
6.0
QED
0.89
Structural alerts
0
No structural alerts detected
Related compounds
Custom attributes
(extracted from source data)
Target Type
Cell Biology
Pathway
chromatin other
Apoptosis
Autophagy
Chromatin/Epigenetic
Target
BRD2, BRD3, BRD4
Bromodomain-containing protein 2
Bromodomain-containing protein 3
Bromodomain-containing protein 4
Bromodomain testis-specific protein
BRD4
BET inhibitor
BRD2, BRD3, BRD4, BRDT
Epigenetic Reader Domain
BRD2
BRD3
BRDT
BRD2, BRD4, BRD3, BRDT
Primary Target
Bromodomains
MOA
Inhibitor
BRD2/3/4/T BET familiy inhibitor
Bromodomain inhibitor
Member status
member
Target subclass
Bromodomain
Bromodomain, Bromodomain, Bromodomain, Bromodomain
Target class
Epigenetics
Epigenetic, Epigenetic, Epigenetic, Epigenetic
Orthogonal probe
JQ1
Recommended Cell Concentration
1 uM
Source data
